SPASMOLYTIC ACTIVITY OF CHLOROFORM FRACTION FROM MORINDA MORINDOIDES LEAVES (BAKER) MILNE-REDH. (RUBIACEAE) USED TO TREAT DIARRHOEA IN TRADITIONAL MEDICINE AND ITS ISOLATED CONSTITUENTS
Dr. Cimanga Kanyanga R.*, Vlietinck A. J. and Pieters L.
ABSTRACT
The present investigation reported the spasmolytic activities of aqueous extract, isolated flavonoids and iridoids form Morinda morindoides leaves which can explain in part, its use as antidiarrhoeal remedy in traditional medicine. Results from this evaluation activity revealed that chloroform soluble fraction, isolated flavonoids and iridoids induced significant acetylcholine (ACh) and depolarizing solution rich in KCl (DSR-KCl) inhibition of isolated guinea-pig ileum contractions by more than 65% at a tested concentration of 40 μg/ml in organ batch. The most active sample was chloroform soluble fraction producing 97.35% inhibition of ACh and 85.30±0.05% of DRS-KCl induced contractions of isolated guinea-pig ileum. Flavonoids chrysoeriol, luteolin, kaempferol and iridoids gaertneric acid, actylgaertneroside, epoxymethoxygaertneroside, dehydrogaertneroside, dehydromethoxygaertneroside, gartneric acid and methoxygaertneroside produced 70 < % inhibition < 80% and 65 < % inhibition < 76% of contractions induced by ACh and DSR-KCl respectively on isolated organ. These results partly suggested that aqueous extract of M morindoides can act as antidiarrhoeal agent by the spasmolytic activity of its flavonoids and iridoids.
Keywords: Morinda morindoides, Rubiaceae, leaf, flavonoids, iridoids, spasmolytic activity, cytotoxicity.
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