SCAVENGE RADICAL ABILITY AND INHIBITION OF LIPID AUTOOXIDATION OF EXTRACTS FROM ANTARCTIC YEAST STRAIN SPOROBOLOMYCES SALMONICOLOR AL1 BIOMASS
Ivanka Stoilova*, Marin Georgiev, Dora Trifonova, Stela Dimitrova, Petko Denev, Albert Krastanov
ABSTRACT
In this study three analytical tests were applied for proving the antioxidant activity of the hexane and acetone
extracts from biomass of Antarctic yeast strain Sporobolomyces salmonicolor AL1. Radical absorbance activity is
established by Oxygen Radical Absorbance Capacity method. The acetone extract is fractioned using thin layer
chromatography and torularhodin was isolated which displays activity of 2183.4 μmol TE/g, which activity is 98
times higher than that of the acetone extract and 8.5 times higher than the hexane extract’s one. Other two
analytical approaches, corresponding to different degrees of autoxidation of lipids, where used - formation of
conjugated double bonds and reaction with thiobarbituric acid - indicator of byproducts formation such as
aldehydes and ketones. At the highest peak of lipid oxidation (at the process’ second day) which corresponds to
the maximum formation of the primary and secondary products, torularhodin inhibits 32.9±0.1% of the chain
reactions as well as the formation of secondary products by 33.0±0.1 %. With the increase of the time for
lipoperoxidation (day 5) turolarhodin inhibition of the chain oxidizing reactions and formation of secondary
products are increased to 52.0±0.1% and 46.0±0.1% respectively. The inhibitory effect of the hexane and acetone
extracts is significantly less than that of torularhodin in both stages of lipid autooxidation.
Keywords: S. salmonicolor AL1, Antioxidant activity , L-ORAC, conjugated diene formation, TBARS.
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