INVITRO CHOLESTEROLESTERASE INHIBITORY ACTIVITY AND CARDIOPROTECTIVE ACTIVITY OF LAWSONIA INERMIS LINN FLOWERS AGAINST ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION IN RATS
Benito Johnson D.*, Santhosh Raj T., Shiny M., Venkatanarayanan R. and Sivasakthi R.
ABSTRACT
Myocardial Infarction is defined as myocardial cell death due to prolonged ischemia. Clinically, the term Myocardial Infarction is used when there is evidence of myocardial necrosis in a clinical setting, consistent with acute myocardial ischemia and with a rise in cardiac troponin. The chemical constituents isolated from flowers of L. interims are Bi-coumarin, Bioflavonoids, Bi-Quinone compounds. The study was evaluating the myocardial infarction activity of ethanol extract of the flowers of Lawsonia inermis Linn in experimental animals. Fresh flowers of Lawsonia inermis L. were cleaned and air-dried at room temperature, pulverized; and were used for extraction with ethanol in soxhlet apparatus. A 1500 g of the pulverized sample was extracted in L ethanol. The extract obtained was collected and concentrated by using rotary evaporator and the percentage yield was calculated. Extract was kept in air tight containers in a refrigerator until used. The results of the present study show a considerable in vitro study of cholesterol esterase inhibitory activity (IC50 = 26±30 μg/ml compared with IC50 = 35±3.350 μg/ml [Atrovastatin]) and in vivo method of grade for cardiac damage for different group of rats using L. inermis extract, so that the extract diminished the myocardial, serum lipid levels and total protein levels in rats. Administration of ethanolic extract of Lawsonia inermis Linn flowers potently protects the myocardium against isoproterenol induced infarction and suggest that the cardio protective effect.
Keywords: Myocardial Infarction, Lawsonia inermis Linn, ethanolic extract, Bioflavonoids.
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