European Journal of Biomedical and Pharmaceutical Sciences

TARGETING OF ANTICANCER DRUG IMPROVES THERAPEUTIC RESPONSE OF ASCITES DALTON’S LYMPHOMA TUMOR IN ANIMAL MODEL

G. Renuga*

ABSTRACT

Poly (lactide-co- glycolide) has gained attention for preparation of variety of drug delivery systems. In the present work PLGA were employed for the preparation of microparticles as controlled drug delivery and drug targeting, focused to explore the preparation of PLGA microparticles containing carboplatin as model drugs. Carboplatin microparticles were prepared by single and double evaporation method, solvent diffusion and the physicochemical properties such as particle size, drug entrapment efficiency, drug loading capacity and yield content were analyzed. In-vitro drug released were measured verses time factor. The incorporation efficiency of carboplatin was higher with the single emulsion evaporation method and the loading efficiency of drug in PLGA microparticles was determined. Drug was administration in to the tumor induced experimental animals as per stated in the methods. The efficiency of drug release form prepared formulae was studied using in vivo technique with Ascites Dalton‟s lymphoma tumor induced animal model. Glutathione–S-transferase (GST) activities are playing significant role in development of resistance in the presences of tumor under various newly developed platinum derivative factors. Western blot analysis showed induction in GST expression in microsphere loaded drug treated samples confirmed carboplatin‟s anticancer activity leading to tumor regression and the ascites was completely diminished.

Keywords: Animal model, Carboplatin, Dalton?s lymphoma tumor, drug loaded, PLGA.