European Journal of Biomedical and Pharmaceutical Sciences

ASSESSMENT OF THE AMELIORATIVE POTENTIALS OF DICHLOROMETHANE EXTRACT OF MUSA ACUMINATA CAVENDISH BRACT IN INDOMETHACIN ADMINISTERED WISTAR RATS

Reginald C. Ohiri, Benjamin A. Amadi and Rukayat Bello*

ABSTRACT

This study investigated the ameliorative potentials of dichloromethane extract of Musa acuminata Cavendish bract in indomethacin administered Wistar rats. Forty five adult male Wistar rats (120-180g) were divided into five groups of nine rats each and a single dose of Indomethacin (40mg/kg bodyweight) was orally administered to study groups and control. Concentrations of 100mg, 150mg and 200mg/kg bodyweights dichloromethane extract of M. acuminata Cavendish bract were orally administered to the animals for three weeks. Dichloromethane extract of sample bract was analysed by Gas chromatography-mass spectrometry (GC-MS) screening while faecal occult blood (FOB) of rats was carried out using immunochromatographic method. The effect on some biochemical indices, hematological indices and histopathology of kidney, stomach and liver of rats were analysed. GC-MS analyses showed the presence of fatty acids; n-Hexadecanoic acid, 9,12-Octadecadienoic acid and 9-Octadecanoic acid as the predominant compounds in the sample analysed. FOB tests were positive (+) at week 0 and week one, then negative (-) at weeks two and three in all treated groups. Compared to the control (21.70 ± 0.12%, 7.24 ± 0.04g/dl, and 3.75 ± 0.05x109/L), a significant dose-dependent increase (p≤ 0.05) in packed cell volume, hemoglobin and red blood count was observed in treated groups (36.80 ± 0.17%, 12.27 ± 0.06g/dl and 5.70 ± 0.11 x109/L) respectively. Serum levels of Aspartate-aminotransferase, Alanine-aminotransferase, Alkaline-phosphatase, creatinine and urea showed a dose-dependent decrease significantly at (p≤ 0.05) in treated groups compared to the untreated control. Histology examination of tissues showed no degenerative changes in study groups. The study confirmed that M acuminata Cavendish at 100mg, 150mg and 200mg/kg improved hematological indices of rats and had no deleterious effect on the liver and kidney tissues of Wistar rats administered indomethacin.

Keywords: Gas chromatography-mass spectrometry (GC-MS), Musa acuminata cavendish bract, indomethacin, gastric injury, faecal occult blood, liver and kidney function.