European Journal of Biomedical and Pharmaceutical Sciences

ANTIOXIDANT EFFECTS OF AQUEOUS EXTRACT OF BLACK MUSTARD (BRASSICA NIGRA) ON THE STOMACH OF WISTAR RATS INTOXICATED WITH PHENYLHYDRAZINE

Edobor Obayuwana* and Samuel Monday Nweke

ABSTRACT

Phenylhydrazine is a chemical intermediate utilized in various industries around the world. It has been implicated in the generation of reactive oxygen species in several studies, while Brassica nigra has been reported to possess strong antioxidant activity. As a result, this study evaluated the antioxidant activity of Brassica nigra seed extract on phenylhydrazine-induced oxidative stress in the stomach of Wistar rats. Aqueous seed extract of Brassica nigra was prepared in the Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin City and thirty (30) albino male Wistar rats weighing between 180 and 250 g were used during this study. Group A received 1 ml distilled water only, Group B received 10 ml of phenylhydrazine only, Group C received 10 ml of phenylhydrazine and 0.1 ml of oxaliplatin, Group D received 10 ml of phenylhydrazine and a low dose of mustard seed (150 mg/kg body weight), Group E received 10 ml of phenylhydrazine and a moderate dose of mustard seed (300 mg/kg body weight) while Group F received 10 ml of phenylhydrazine and a high dose of mustard seed (600 mg/kg body weight). All administrations were done by gavage and lasted for eight (8) months, after which, the rats were sacrificed, tissues harvested, and appropriate histological and biochemical investigations were carried out. Results showed that phenylhydrazine significantly increased levels of MDA, while simultaneously significantly reducing levels of GPx and SOD. Treatment with B. nigra attenuated these biochemical effects while similar positive results were obtained from the histology. This study suggests that B. nigra is an important physiological antioxidant that can help to decrease oxidative damage induced by phenylhydrazine in gastric tissues.

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