European Journal of Biomedical and Pharmaceutical Sciences

METHOD DEVELOPMENT, VALIDATION AND SIMULTANEOUS ESTIMATION OF QUINAPRIL AND HYDROCHLORTHAIZIDE IN TABLET DOSAGE FORM BY USING RP-HPLC

Sumalatha Chepyala* and G. Teja

ABSTRACT

A simple, accurate, precise method was developed for the simultaneous estimation of the Quinapril and HCTZ in Tablet dosage form. Chromatogram was run through Inertsil ODS column (250mm: 4.6mm, 5μ). Mobile phase containing Buffer & Acetonitrie in the ratio of (45:55) was pumped through column at a flow rate of 0.8ml/min. Buffer used in this method was ortho phosphoric acid Temperature was maintained at 30°C. Optimized wavelength for Quinapril and HCTZ was 210nm. Retention times of Quinapril and HCTZ were found to be 2.17min and 3.4min. % RSD of the Quinapril and HCTZ were and found to be 0.65 and 0.42 respectively. % Recover was Obtained as 99.91 and 100.02 for HCTZ and Quinapril respectively. LOD, LOQ values are obtained from regression equations of Quinapril and HCTZ were 0.67, 2.03 and 0.5, 1.52 respectively. Regression equation of HCTZ is y = 20044x + 4078, and of Quinapril is y = 317877x + 2711 Regression co-efficient was 0.999.

Keywords: HCTZ, Quinapril, RP-HPLC.