IMPLICATIONS OF CANNABIS SATIVA EXPOSURE ON PATTERN OF ADVANCED OXIDATIVE PROTEIN PRODUCTS (AOPP), PAIN HYPERSENSITIVITY AND MEMORY IN ADULT WISTAR RATS
Ibeachu C. P. and *Olorunfemi O. J.
ABSTRACT
The present study was carried out to investigate the implications of cannabis sativa exposure on pattern of advanced oxidative protein products (AOPP), pain hypersensitivity and memory in adult Wistar rats. This was achieved by determining the time of retraction of the experimental animals over a period of three weeks, analysis of oxidative parameters and advanced oxidative protein products. Twenty-five (25) healthy Wistar rats weighing 180-240g were used for this study. They were divided into five (5) groups of five (5) animals each. Group one serve as the normal control group, and was given distilled water, group two was given 0.5ml of cannabis, group three was given 0.7ml of cannabis, group four was given 1ml of cannabis and group 5 was given epinephrine. All administrations lasted for three weeks, during which time, the experimental animals were subjected several tests. Pain sensitivity threshold was determined using the analgesy-meter device with each group undergoing three trials. Passive avoidance test was used to determine the effect of cannabis on memory and spatial recognition in light and darkness. At the end of the administration period, brain specimen of experimental animals were harvested and homogenized and the quantification of oxidative stress parameters such as malondialdehyde, catalase, superoxide dismutase, glutathione reductase and AOPP were carried out. The data obtained was analyzed using Statistical Package for Social Sciences (SPSS version 23). Results obtained showed that sub-acute exposure of cannabis led to reduction in the levels of AOPP in brain tissues. It also revealed in a decrease in the level of oxidative stress parameters such as glutathione reductase, superoxide dismutase and catalase while an increase was seen in malondialdehyde. It also led to some level of reduction in the retraction time indicating an increased sensitivity to pain.
Keywords: Cannabis, OAPP, memory, pain hypersensitivity, oxidative stress parameters.
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