European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO ANTI INFLAMMATORY ACTIVITY OF PTEROSPERMUM RUBIGINOSUM BARK EXTRACT BY USING ALBUMIN DENATURATION METHOD

Vijeesh Govindan* and U. Srinivasa

ABSTRACT

Natural components present in any natural source have the potential to inhibit the pathways responsible for causing inflammation. It has gained huge demand as they tend to possess lesser side effects. Pterospermum rubiginosum, belonging to the family, Sterculiaceae, is a plant used in the treatment of bone fracture and its related complications. The use of different parts of P. rubiginosum has been in practice by the traditional people, particularly the tribal communities living in deep interior forests. However, only a little research has been performed on this drug to document any of its therapeutic capabilities. The present research was done with this in view and in an attempt to study anti-inflammatory potential by invitro protein denaturation activity. Different solvent extracts of the bark obtained by soxhelation method were tested for the activity. The concentration gradient of each extract was prepared using bovine serum albumin and phosphate buffered saline. These extracts were adjusted to pH 6.3 and incubated at 37oC for 20 minutes. Diclofenac sodium was used as standard and the percentage inhibition of protein denaturation was calculated. The inhibition rate of bovine serum albumin denaturation for all the solvent extracts increased gradually with an increase in concentration. A commendable high rate of inhibition was observed in the ethanol extract compared to others which indicated the anti-inflammatory potential of the bark part of the plant that increases with the concentration of the extract.

Keywords: Anti-inflammatory, bovine serum albumin denaturation, ethanolic extracts.