European Journal of Biomedical and Pharmaceutical Sciences

TO STUDY WASHING EFFICACY OF MEHNDI, AALTA, DRY SINDOOR, WET SINDOOR STAINED FABRIC WITH DIFFERENT EMULSIFIERS MEDIATED PEARL MILLET AMYLASE BOUND BSA NANOPARTICLES AS DETERGENT-ADDITIVES

Kirti Rani*, Sunidhi Kant, Shreya Goyal and Chandrika Gupta

ABSTRACT

Catalyzing property of amylase has been exploited in textile and detergent industries for desizing and stain removal. Amylase is stabilized via immobilization onto bovine serum albumin (BSA) to prepare their fabricated nanoparticles. In this work, we extracted amylase form Pearl millet and bound in BSA nanoparticles with four Almond Oil, Jasmine Oil, Mustard Oil, and Olive Oil as naturally occurring emulsifier which don’t have any toxic effect. These fabricated nanoparticles were used as bio-active detergent additives with different detergents named, Ariel matic, Surf excel liquid, Active wheel and Tide plus. These were tested for their stain removal competency to get washing efficacy. The stains slected in this study were comprised of cosmetic stains, namely, Mehndi, Aalta, Dry sindoor, Wet sindoor. These traditional or festive cosmetics stains are tough to wash off and often require pre-washing practice. Hence, effective comparative study was carried out from this study and all strains are very well vanished if when washed with chosen detergents with the prepared enzyme bound nanoparticles mixture as compared to when washing was done with chosen strains only. Among them, Ariel matic detergent with enzyme loaded bovine serum albumin nanoparticles was found to be the best washing bio-active system as compared to other chosen detergent samples, followed by Surf excel liquid, Tide plus and the least effective was found to be Active wheel. Among the prepared enzyme bound BSA nanoparticles, Almond and Olive oil driven nanoparticles was showed excellent washing efficacy when coupled with Ariel matic while mustard oil nanoparticles with Ariel matic also showed very good washing results. Furthermore, very good results were also observed when stained cloth washed with Almond and Olive Oil driven nanoparticles with Surf Excel liquid while fairly good results were shown by Jasmine oil driven and Mustard Oil driven nanoparticles followed by Almond and Olive Mustard Oil driven nanoparticles with Active wheel and tide plus. And, least washing efficiency was found to be for Jasmine oil driven BSA nanoparticles.

Keywords: Mehndi, Aalta, Dry sindoor, Wet sindoor; Almond oil; Olive oil; Jasmine Oil; mustard oil; BSA nanoparticles; Detergents.