Abstract
NOOTKATONE AND ANTIOXIDANT COMPOUNDS IN INOCARPUS FAGIFERUS FOSB SEEDS

Sri Rahayu Santi*, I. Made Sukadana and Yosi Rama Dhani

ABSTRACT

Inocarpus fagiferus Fosb or Gayam is one of the species of the Legumineceae family whose seeds have been traditionally used for anti-inflammatory and coronary heart disease caused by plaque atherosclerosis. Gayam seeds in vivo potentially can be used as an antioxidant regarding atherosclerosis mechanism. This study aims to isolate and identify antioxidant activity toward DPPH of Gayam seeds. The result of antioxidant activity showed that IC50 values 156.00; 230.00; and 1303.76 mg/L of n-hexane, chloroform, and water extract respectively. Separation of n-hexane extract using column chromatography (stationer phase silica gel 60; mobile phase n-hexane-chloroform (2:1)) produced 4 fractions with the smallest IC50 value in fraction C being 330.24 mg/L. Analysis of mass spectra from the chromatography peaks of LC-MS/MS result showed that the isolate (fraction C) allegedly contains nootkatone as an antioxidant compound which is a mixture with other compounds (22E)-chola-5,22-dien-3-ol; 5,7-dimethoxyflavanone; (11β,16α)-11,17,21-trihydroxy-16-methylpregn-4-ene-3,20-dione; 11β-hydroxy-3,20-dioxopregn-4-en-21-oic-acid and unidentified compound with a mass of 321.0792 g/mol.

Keywords: Antioxidants, atherosclerosis, Inocarpus fagiferus Fosb, LC-MS/MS, and Nootkatone.


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