ISOLATION, CHARACTERIZATION AND IDENTIFICATION OF POTENT LIPASE PRODUCING BACTERIA FROM OIL SPILLED SOIL OF OIL PROCESSING FACTORY IN BURDWAN, WEST BENGAL
Indrani Paul, Abhijit Sar and Somasri Dam*
ABSTRACT
Lipases are glycerol ester hydrolases that catalyze the hydrolysis of triglycerides to free fatty acids and glycerol.
Lipases have great importance due to their wide usage in industry. Lipases are produced by microorganisms
(bacteria and fungi), plants and animals. However, microbial lipases, especially from bacteria are more useful than
their plant and animal derivatives because of several important properties. The primary goal of this study is to
isolate and characterize novel lipase producing bacteria from soil sample. We have selected oil spilled soil in
Burdwan, West Bengal, India to isolate and characterise novel lipase producing bacteria. The characterisation
includes morphological and biochemical characterization of isolates, determination of the enzyme activity and
effect of temperature on the growth of the isolates. From biochemical and morphological analysis we found that
all isolates were gram positive rods, endospore former, non-citrate utiliser, non-reducer of sulphur compounds etc.
From 16s rRNA sequencing four of them was found to be Bacillus. To understand the evolution of lipase proteins,
we have done a phylogenetic tree with the amino acid sequences from among diverse group of organisms.
Keywords: Lipase producing bacteria, oil factory, Bacillus.
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