European Journal of Biomedical and Pharmaceutical Sciences

ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE T-786C POLYMORPHISM AMONG SICKLE CELL ANAEMIA PATIENTS IN KHARTOUM, 2017

Hiba Hussein Tanoon* and Ibrahim Khidir Ibrahim

ABSTRACT

Background: Sickle-cell disease (SCD) is a group of genetically passed down blood disorders. Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. Objective: To detect Endothelial Nitric Oxide Synthase Gene T-786C polymorphism among Sudanese patients with Sickle cell disease. Materials and Methods: Ninety two samples included in this study, 46 of them were sickle cell disease Sudanese patients and other 46 as a control group were evaluated to detect Endothelial Nitric Oxide Synthase Gene T-786C polymorphism using Polymerase Chain Reaction. Results: The molecular analysis showed that the detected genotype in sickle cell anaemia patients was T/T; as in the control group the T/T genotype, while the genotype T/C and C/C was completely not detected among the study subjects. No association was found between SCA and presence of nos3 gene polymorphism it was not a risk factor for the development of SCA. Conclusion: The present study showed no associations between the eNOS SNP (T-786C) and sickle cell anaemia in Sudan.

Keywords: Sudanese, Nitric Oxide Synthase, Polymorphism, Sickle cell disease.