SIMULTANEOUS ESTIMATION OF 11-KETO-?-BOSWELLIC ACID AND WITHAFERIN-A BY USING HPTLC METHOD FROM PAINQUIT TABLET
Unmesh Keshwar*, Soumit Pimplapure, Sharad Dhurde and B. K. Shrikhande
ABSTRACT
Background: Herbal medicines are widely used for the management of pain and inflammation. Most of the herbal formulations present in the market are not standardized with respect to its active ingredients marker molecule. Siddhayu Ayurvedic Research Foundation Pvt. Ltd. has developed and validated Painquit Tablet to be used for pain reliving, inflammation, mobility, flexibility and strength. Objective: The present study was designed with an objective of simultaneous estimation of 11-Keto-β-Boswellic acid and Withaferin A by HPTLC from Tablet containing Boswellia serrata and Withania somnifera. Methods: High performance Thin Layer Chromatography (HPTLC) method was developed and validated for simultaneous estimation of 11-Keto-β-Boswellic acid (KBA) and Withaferin A (WA) from Painquit Tablet. Chromatographic separation was achieved on precoated silica gel HPTLC aluminium plate 60 F254 using Chloroform: Ethyl Acetate: Formic acid, 7.0:3.0:1.0 (v/v/v) as mobile phase followed by densito-metric measurement at two different wavelength 258 nm and 227 nm by TLC Scanner 4 (CAMAG).The method was validated according to International Conference on Harmonization guidelines. Results: The retention factors of KBA acid and WA were 0.58 and 0.32 respectively. The estimated amount of KBA and WA was found to be 0.15 % and 0.09 %. Results obtained in validation parameters indicate the accuracy and reliability of the developed simultaneous HPTLC method for the quantification of KBA acid and WA in Painquit Tablet. Conclusion: The proposed method found to be simple, sensitive, selective and accurate for routinely used in Quality Control Lab.
Keywords: 11-Keto-?-Boswellic acid, Withaferin A, Painquit Tablet, HPTLC, pain reliving, inflammation.
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