European Journal of Biomedical and Pharmaceutical Sciences

PRODUCTION, PURIFICATION AND OPTIMIZATION OF MEDIA FOR ALKALINE PROTEASE FROM BACILLUS SUBTILIS MTCC1028.

Shipra Kalra*, Kanav Midha and Amandeep Singh

ABSTRACT

The main aim of this study was production, partial purification and media optimization of alkaline protease enzyme by Bacillus subtilis MTCC 1028. Bacillus subtilis was allowed to grow in broth culture for purpose of producing protease enzyme. Alkaline Protease production by Bacillus subtilis was performed with optimum substrate concentrations, optimum incubation temperature, optimum pH, optimum carbon sources and optimum nitrogen source for protease production. The protease enzyme was purified by Ammonium Sulfate precipitation. A trial for the purification of protease resulted in an enzyme with specific activity of 25 (IU/mg). The protease activity increased as the increase in enzyme concentration with optimum substrate concentration. Wheat bran optimum incubation temperature was 37 ºC. Purified protease enzyme had a maximum activity at pH 8.0 of phosphate buffer, with the optimum incubation time of 72 h.

Keywords: Proteases, enzyme, purification, ammonium sulphate.