Anjali Nair G.*, Anjali Murali, Devika Suresh, Farsena Tabassum, Mariya Paulson and Shahin Mohammed K.V.


Aim: The present study was to standardize and evaluate antibacterial, antioxidant and invitro antiarthritic activity of Maharasnadi Kashayam (MRK). Methodology: The formulation was physicochemically and microbiologically standardized. Standardization parameters like organoleptic characters, physicochemical analysis, phytochemical analysis and sterility test were carried out as per Ayush guidelines. Antibacterial studies were carried out by using agar well diffusion method. In vitro antioxidant study such as DPPH assay was performed and was compared with standard ascorbic acid. Invitro antiarthritic activity of MRK was performed by using egg albumin protein denaturation method. Results and discussion: The physicochemical parameters was performed and the result were found to be in permissible limits. Antibacterial studies showed significant zone of inhibition against Bacillus subtilis, Pseudomonas fluorescens, Staphylococcus aureus and Lactic acid bacillus. Among the bacterial strains, P. flurescens shows highest zone of inhibition. Maharasnadi kashayam (MRK) showed comparable antioxidant potential by DPPH assay method when compare to standard ascorbic acid and IC50 value found to be as 13.11and 22.81μg/ml for ascorbic acid and Maharasnadi kashayam (MRK) respectively. The activity of MRK was compared with standard Diclofenac sodium. MRK showed considerable inhibition of protein denaturation compared to that of standard. Conclusion: The outcome of this study clearly proves the quality, purity, safety and potency of the drug which will help the medicine to survive and succeed in future researches on both clinically and economically.

Keywords: Antioxidant, Antiarthritic activity, Standardization, Maharasnadi Kashayam, Ascorbic acid, Diclofenac sodium.

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