Abstract
ASSESSMENT OF PHYTOCHEMICALS, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Bixa orellana LEAF

Garima Dhanawat, Tanushree, Chatterjee, Virendra Vaishnav*, Sheetal Devtare and Dugeshwar Karley

ABSTRACT

Bixa orellana is a well known plant in Asia, including India, which has a wide range of medicinal importance. The drugs of this plant are used in India as a folk remedy in the form of decoctions and infusions to treat bacterial infections and it is also effective against different skin diseases. The present study is to examine the bioactive compounds, Antimicrobial properties and antioxidant activity of leaves extracts of Bixa orellana with ethanol solvent. Phytochemicals such as Alkaloids, Flavonoids, phenols, carbohydrates, cardiac glycosides and saponins were recorded positive result in ethanol extract. Antibacterial activity of ethanolic extract was tested against Pseudomonas aeuroginosa, by well diffusion method to study the presence of various phytochemicals in Bixa orellana then the zone of inhibition 12.7 mm was calculated. Antifungal activity of ethanolic extract were tested against Collectotrichum acutatum, Pemicillium griseofulvum, Alternaria alternate and Rhizopus oryza. The leaves showed significantly higher moisture, flavonoids, phenols and saponins content; hence to ensure sustainable management of the plant resources, the leaves should be the primary target of any phytochemicals extraction activities for B. orellana.

Keywords: Phytochemical, Flavonoids, Antibacterial, Antifungal, Bixa orellana.


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