EFFECT OF SALICYLIC ACID ON INCREASING PHENOLIC COMPOUNDS OF CATHARANTHUS VINCA (L.) G. DON. USING SHOOT TIP IN VITRO
Merfat A. Abdalrahman Al-Karkhi and *Hashim K. Mohammed Al-oubaidi
ABSTRACT
Bioactive compounds in plants can be defined as plant secondary metabolites using in pharmacological for human healthy. Phenol compounds are produced within the Catharanthus vinca. Plants besides the primary biosynthetic and metabolic routes for compounds associated with plant growth and development. Several of them are found to hold various types of important functions in the treatments but this medicinal compounds are produced in little amount in plant so it's necessary to stimulate the plant to increase the amount of production. The present research was conducted in order to increase some secondary metabolites Phenolic compounds of Catharanthus vinca. In vitro by adding different concentrations of Salicylic acid (50,100,150 or 200) mg/l to Shoot tip media then this compounds are estimated by high performance liquid chromatography (HPLC) and compared with those in mother plant. The results showed that the concentrations (200,150 or 100) mg/l of Salicylic acid for increasing Phenolic compounds and cause highly significant differences.
Keywords: Catharanthus vinca, Salicylic acid.
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