Abstract
ISOLATION AND CHARACTERIZATION OF EMODIN IN CASSIA MIMOSOIDES LINN.

Dr. Minu B. Pattasseril*, Dr. Sujith Abraham, Aleena V., Amna Sherin U. and Jinsha K. P.

ABSTRACT

Herbal medicine is making a dramatic comeback as the side effect of synthetic medicine are daunting and the therapeutic approach is drifting towards substituent medicine. Emodin is a natural anthraquinone derivative that occurs in many widely used Chinese medicinal herbs, such as Rheum palmatum, Polygonum cuspidatum and Polygonum multiflorum. Emodin has been used as a traditional Chinese medicine for over 2000 years and is still present in various herbal preparations. Emerging evidence indicates that emodin possesses a wide spectrum of pharmacological properties, including anticancer, hepatoprotective, antiinflammatory, antioxidant and antimicrobial activities. Cassia or Chamaecrista mimosoides is an annual or occasionally a short-lived perennial plant with a more or less woody base. The stems can grow up to 1 metre long. The root of this plant is used in diarrhea and spasm of stomach. Pulverized leaves are used for dressing wounds, sores, mouth ulcers, spasm of stomach and headache. Cassia mimosoides leaves and pods as a potential agent in vector control of malaria and to evaluate repellent effect against Anopheles gambiae mosquito. Phyochemical screening using Borntranger's test showed the presence of anthraquinone. Physical and chemical tests of isolated compound was conducted. Preparative TLC of the ethyl acetate extract using Benzene-Ethyl Acetate -Acetic Acid (75:24:1), at Rf value 0.97, Emodin was isolated. By using IR, spectra of the isolated compound were confirmed.

Keywords: CASSIA MIMOSOIDES LINN.; Phytochemical screening, anthraquinone glycoside.


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