Abstract
ANALYTICAL DEVELOPMENT AND EVALUATION OF BHUTRIN GHRITA

Dr. G. N. Pramodini*, Syed Hafeez Ahmed, Dr. Shaik Mohd Khasim, Mohd Rashid and Md. Mohiuddin

ABSTRACT

Bhutrin ghrita (BG) is a polyherbal Ayurvedic formulation and it contain Lemon Grass. In Ayurveda it is called as Bhutrin, Bhrami, Giloy, Harda, Cow Ghee. As Ghee is a 100% lipid which enhance its absorption into the body cell. As lemon grass is a very good antioxidanting agents and bhrami is a Brain tonic and Giloy and Harda helps to increase and maintain the immune system. So, the main aim and objective of this research work is to formulate a unique Ayurvedic formulation. Which can be used in ageing and related ailments and to maintain good health. In present research work, an attempt was made to develop an Ayurvedic Formulation which is a cow ghee based Polyherbal Bhutrin Ghrita and evaluation of It was done Pharmacognostically and Phytochemically and to develop an Analytical profile of the Bhutrin Ghrita. The plants under the study were identified procured, authenticated and processed. The fresh plants juices of all the plants materials were prepared and used for the developments of polyherbal Bhutrin Ghrita as per Ayurvedic procedures. The prepared Ghrita formulation were subjected to organoleptic (colour, odour, taste, appreance and touch), Physicochemical Parameters like Acid values, Saponification, and iodine value was done. HPTLC Studies were performed to know the standard of the formulation. The prepared Ghrita formulation were subjected to Pharmacognostical evaluation like Powdered characters identification and organoleptic evaluation (colour, odour, taste, appreance and touch), were performed to overcome adulteration followed by Physicochemical evaluations like Acid values(4.26 0.26 mg KOH/gm), Saponification (22.7 0.23 mg KOH/gm), and iodine value (34.75 0.002 mg KOH/gm) was done the results of this are expressed as mean SEM all analysis were done in triplicate. HPTLC - Fingerprint Studies were performed to know the standard of the formulation. The results of all the Physicochemical and Pharmacognostical evaluations were in the Ayurvedic standard limits. In HPTLC - Fingerprint Studies it was identified that the Polyherbal Bhutrin Ghrita Formulation may contains chemical constitutes, for Brahmi the Rf value was 0.05 which may be Bacopaside, in Harda the Rf value was 0.95 which may be Ellagic Acid and Gallic Acid, in Lemongrass the Rf value was 0.02 which may be Citral with and in Giloy the Rf value was 0.1 which may be Cardifolidin. So, the Polyherbal Ghrita formulation was confirm to have all the chemical constituents which are present in a mixed form. The Bhutrin Ghrita may be used as a memory enhancer, immunity boosting agent and as a good antioxidanting Ayurvedic polyherbal formulations.

Keywords: Bhutrin Ghrita, Pharmacognostical, Phytochemical.


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