European Journal of Biomedical and Pharmaceutical Sciences

DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF B-SITOSTEROL IN LEAVES OF SOLANUM XANTHOCARPUM SCHRAD & WENDLE

Shaik Rafiya, Ajmera Rama Rao and Ciddi Veeresham*

ABSTRACT

Solanum xanthocarpum is an important medicinal plant belonging to the family Solanaceae. β-sitosterol is a main constituent of solanum. A simple, rapid, sensitive, accurate, repeatable and robust HPTLC method has been developed and validated for the estimation of β-sitosterol in the leaf extract of Solanum xanthocarpum. β-sitosterol was estimated at 206 nm by densitometry using Silica gel 60 F254 as stationary phase and Chloroform: Toulene (9.5:0.5),and as mobile phase. Linearity was observed in the concentration range 20-120ng/spot for β-sitosterol. The limit of detection and limit of quantification were found to be 0.03 & 9.17ng/spot respectively for β-sitosterol. Developed method was validated according to the ICH guidelines with respect to precision, accuracy, specificity and robustness. The technique has been applied for the first time for the estimation of β-sitosterol in leaf extracts of Solanum xanthocarpum. Statical analysis data indicate the accuracy and reliability of the method.

Keywords: Solanum xanthocarpum, ?-sitosterol, HPTLC, and Validation.