European Journal of Biomedical and Pharmaceutical Sciences

EXTRACTION, CHARACTERIZATION, ANTIMICROBIAL APPLICATIONS OF COLLAGEN FROM ALBINO WISTAR RAT TAIL TENDONS (LABORATORY WASTE)

Hemambujam Devanathan*

ABSTRACT

Collagen is the abundant protein in various connective tissues to animal proteins and has been widely used as application materials for tendons, bones and muscles. A rat tail tendon is among the original source of Type I collagen and it was observed that mature female rats give best yield than male and immature rats. The specific purpose of the present study was to extract and analyses collagen from Albino Wistar rat tail tendons. Albino Wistar rat tail tendons were collected and stored at -20°C. Tendons were removed from tails then tendons were treated with NaCl to remove non-collagenous tissue. Then, were solubilized using acetic acid method. The solubilized collagen was precipitated by using NaCl and dialysis was carried out. Then SDS-PAGE was done to separate proteins according to their molecular weight and UV analysis was performed. The objectives of this present study is to assess the use of Albino Wistar rat tail tendons (laboratory waste) as a source of collagen and its extraction, characteristics and to check its antifungal and wound healing activity. The total amount of collagen that was extracted from 20.90g of rat tendons was 12ml of collagen. Rat tail collagen showed significant in Antifungal activity and based upon the results of this study, collagen showed broad application in wound healing activity.

Keywords: Rat tail tendons (laboratory waste), Type I collagen, non-collagenous tissue, solubilized and precipitated collagen, dialysis membrane, SDS-PAGE, UV analysis, antifungal activity, wound healing activity.