European Journal of Biomedical and Pharmaceutical Sciences

ISOLATION AND CHARACTERISATION OF BIOACTIVE COMPONENTS FROM PHASEOLUS COCCINEUS SEEDS FOR ANTIOXIDANT ACTIVITY

Vaishali Soni*, Dr. Brajesh Kumar Arjariya, Rahim Khan, Sheenam Mansuri and Ankit Mehra

ABSTRACT

Historically, all medicinal preparations were derived from plants, whether in the simple form of plant parts or in the more complex form of crude extracts, mixtures, etc. The present study is aimed at evaluating the antiurolithiatic activity of the ethanolic extract and aqueous extracts of the seeds of Phaseolus coccineus. The seeds of Phaseolus coccineus Linn were collected from farmers of Ooty, Tamil Nadu, India. Coarse powder of Phaseolus coccineus Linn seeds (300 grams) were extracted by cold maceration using water for 15 days. The percentage yield of ethanolic and aqueous extract of seeds of Phaseolus coccineus linn was found to be Ethanolic extract: 8.24 % w/w. Aqueous extract: 6.32 % w/w. Preliminary Phytochemical screening of Phaseolus coccineus, revealed the presence of following phytoconstituents Alkaloids, Carbohydrates, Proteins & amino acids, Steroids, Phenols, Tannins, Flavonoids, Glycosides. Both extracts showed a very good concentration-dependent inhibition of lipid peroxidation and almost equal activity. Total Flavonoid Content of EEPC (9.18 mg) was found to be more compared with the AEPC (8.81 mg). Total Phenolic Content of EEPC (42.22 mg) was found to be more compared with AEPC (30.10 mg). The results of the present investigation on the evaluation of Phytochemical screening and anti oxidant studies of aqueous and ethanolic extracts have led to acceptable findings. Flavonoids, Polyphenols and saponins present in the extracts may be responsible of the antioxidant.

Keywords: Antiurolithiatic, herbal, Phaseolus coccineus, Urinary System, kidney.