Abstract
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PHARMACEUTICAL DRUGS BY RP-HPLC METHOD

Sen Shailesh*, Sharma Ranu, Kachawa Vijay Singh, Garg Ayush and Dwivedi Dr. Jayesh

ABSTRACT

Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A simple, rapid, precise, and reliable reverse phase HPLC method was developed for the separation and estimation of two drugs hydrochlorothiazide and eprosartanin bulk drug mix and pharmaceutical dosage forms. The estimation was carried out using column; mobile phase consisting of methanol, orthophosphoric acid and buffer at pH 5; the flow rate of 1.7 mL/min and ultraviolet detection at 232 nm. All the drugs were properly resolved having run time of 2.5 and 4.3 minutes for hydrochlorothiazide and eprosartan, respectively. The method was validated as a final verification of method development with respect to precision, linearity, accuracy, ruggedness, and robustness. The validated method was successfully applied to the commercially available pharmaceutical dosage form, yielding very good and reproducible result.

Keywords: RP-HPLC, hydrochlorothiazide and eprosartan, method development.


[Full Text Article]   [Download Certificate]

Indexing

Indexing

Best Article Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) is giving Best Article Award in every Issue for Best Article and Issue Certificate of Appreciation to the Authors to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: JUNE ISSUE PUBLISHED

    JUNE 2026 Issue has been successfully launched on 1 JUNE 2026.

  • EJBPS: New Impact Factor 2026

    EJBPS Impact Factor has been Increased from  7.482 to 8.181 for Year 2026.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     
Google Scholar Index Copernicus Indian Science Publications Urlich's Periodicals Directory, Proquest, UK (In Process) Research Bible, Fuchu, Tokyo. JAPAN UDLedge Science Citation Index International Society for Research activity (ISRA) Scientific Indexing Services (SIS) InfoBase Index (In Process) SJIF Impact Factor Scholar Article Impact Factor, SAIF Universal Impact Factor CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Global Impact Factor (GIF) (0.377) IP Indexing (IP Value 3.77) Web of Science Group (Under Process) Directory of Research Journals Indexing Scope Database Academia Academia Doi-Digital Online Identifier Doi-Digital Online Identifier ISSN National Centre Zenodo Indexing International CODEN Service, USA

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT