European Journal of Biomedical and Pharmaceutical Sciences

TRYPANOCIDAL ACTIVITY OF EXTRACTS AND CASSANE DITERPENOIDS ISOLATED FROM THE LEAVES OF ERYTHROPHLEUM SUAVEOLENS (GUILL. ET PERR.) BRENAN (FABACEAE)

Assamoi Vincent Adiko, Molou Guillaume, Coulibali Siomenan, Coulibaly Souleymane, Seri Seri Chardin, Timotou Adeyolé, Konan Dibi Jacques, Kablan Richmond Jean-François, Kabran Aka Faustin*,
Kablan Ahmont Landry Claude and Attioua Koffi Barthélemy

ABSTRACT

Erythrophleum suaveolens is a plant species of ivorian flora used to treat several pathologies including Buruli ulcer, tuberculosis and certain parasitic diseases. To our knowledge, no phytochemical study has been conducted on leaves of this species. This work carried out on the leaves is in line with development of medicinal plants in Côte d'Ivoire through the evaluation of trypanocidal activity of extracts and molecules isolated from different parts of the species. The phytochemical study was carried out using classical chromatographic methods (silica column chromatography, Sephadex column chromatography) and structural determination (NMR, IR, MS). The evaluation of trypanocidal activity against Trypanosoma brucei brucei was performed using the method used by N’Guessan et al.[1] The chemical study led to the isolation of two amide- and amine-functional cassane diterpenoids. These are Nor-cassamine (1) and Norcassamide (2), isolated for the first time from the leaves of this species. Biological tests showed that cold methanolic extract and Norcassamine (1) have trypanocidal activity, even if this remains moderate compared to the reference molecule, Melarsoprol.

Keywords: Erythrophleum suaveolens, Norcassamine, NMR, trypanocidal activity.