Abstract
COMPARE STUDIES OF SOUTHERN –NORTHERN- WESTERN BLOTTING

Tanima Debnath Sarkar* (PhD Scholar) and P. Senthil Elango

ABSTRACT

The extraction of biomolecules, DNA, RNA, and protein, is the most crucial method used in molecular biology.[1] These biomolecules can be isolated from any biological material for subsequent downstream processes, analytical, or preparative purposes.[2] Southern blotting, Northern blotting and Western blotting are the major blotting to find out DNA, RNA and protein respectively. In the world all life are standing in amino acid molecules. Because DNA means deoxyribonucleic acid and main ingredient of nucleic acid is amino acid. Amino acids are the building block of protein and proteins are the long chain of amino acids. There are some important factors of amino acids are charge, hydrophilicity or hydrophobicity, size and functional groups.[3]

Keywords: Amino acids, protein, blotting, hydrophilicity, functional group.


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