Abstract
COMPARATIVE STUDY ON ANTIMICROBIAL EFFECT OF AQUEOUS, ACETONE AND ETHANOL EXTRACTS OF CALENDULA OFFICINALIS L. (MARIGOLD) FLOWER

Priyanka Pandey*, S. Karmakar and Wasim Raja

ABSTRACT

Infectious diseases have always been one of the important concerns of human and have continuously attracted the attention of a large number of various medical and laboratory professionals. On the other hand, treatment with antibiotics has other problems such as drug resistance and side effects, so the use of new herbal medicines with fewer side effects can be a great help in treating these types of infections. The present investigation was carried out to evaluate the antibacterial activities of Calendula officinalis Linn. The effect of aqueous, acetone and ethanol crude extract of petals of flowers in different concentrations were obtained and bioassay in vitro for its bioactivity to inhibit the growth of eight types of bacteria’s gram positive bacteria; Bacillius Subtilis, S. aureus, Enterococcus and gram negative bacteria; P. Aeruginosa, K. Pneumoniae, E. Coli. The extracts of petals part were clearly superior for all bacteria especially the bacteria Bacillius Subtilis (inhibition zone was 20 mm in the concentration 100 mg/ml) from gram negative bacteria, and E. Coli (inhibition zone was 16 mm in the concentration 75 mg/ml) from gram positive bacteria, while the extracts of ethanol was more effective as compared to aqueous, acetone extract. These results confirm the antibacterial activity of Calendula officinalis flowers and support the traditional use of the plant in the therapy of bacterial infection. These findings corroborate the traditional usage of Calendula officinalis flowers in the treatment of bacterial illnesses by confirming their antibacterial activity.

Keywords: Antibacterial, Calendula officinalis, infection, ethanol, Flower Extract.


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