European Journal of Biomedical and Pharmaceutical Sciences

CORRELATION BETWEEN AUTOIMMUNE DISEASES AND EXTRACTABLE NUCLEAR ANTIGEN ANTIBODIES PANEL AMONG PATIENTS AT KING HUSSEIN MEDICAL CENTER

Lamees Abasi (MD)*, Maha Al-Amr (MD), Nabeeha Abbasi (MD), Osama Rabadi (MD), Shadi Aldaoud (MD), Sahel Haddadin (MD), Mu’taz Halasah (MD), Ahmad Mahasna (MD), Ra’ad Al-omari (MD), Huda Khraisat (MD)

ABSTRACT

Objectives: The production of autoantibodies is one of the characteristics of autoimmune diseases (ADs), autoantibodies against extractable nuclear antigens (ENA) are important category of them that could assess the differentiation between various types of autoimmune disorders. The aim of this retrospective study is to confirm the correlation between ENA panel of patients with their clinical diagnoses. Patients and Methods: This study goes over patient’s records in the adult medical clinics at King Hussien Medical Center (KHMC), during the time period between July 2020 and July 2021. The study group included 200 patients, aged between 18 and 80, females were 144 and males were 56. Serum samples were collected from these patients, then were investigated for the presence of anti-ENA antibodies using Immunoblot method. Results: Of the 200 samples, (47%) were positive for anti-ENA. The highest percentage in the study group was anti-SSA/Ro autoantibody which revealed (44%). (29%) of the patients who were negative for anti-ENA, were diagnosed to have an AD, on the other hand, (78%) of the patients who were anti-ENA-positive were diagnosed to have an AD. Systemic lupus erythematosus (SLE) was the highest prevalence in the anti-ENA positive patients. Conclusion: The correlation between anti-ENA antibodies and the diagnosis of autoimmune disease was significant. As a consequence, the anti-ENA antibodies test is a worthy indicator to support in the clinical diagnosis of patients with autoimmune diseases.

Keywords: Autoantibodies, autoimmune diseases, extractable nuclear antigens, panel.