European Journal of Biomedical and Pharmaceutical Sciences

AN ASSESSMENT OF THE CURATIVE IMPACT OF A POLYHERBAL REMEDY JALIN FOR CYCLOPHOSPHAMIDE-INDUCED TOXICITY IN MALE ALBINO RATS' LIVER

Arhoghro Ejovwoke Marcellinus*, Berezi E. P. and Owotgwun Kasirotu Levi

ABSTRACT

This study aimed to evaluate cyclophosphamide induced hepatotoxicity ameliorated by Jalin herbal extract (Ginseng, Lepedium meyenii, Liriosma ovata and Honey). Fifteen (15) albino male rats were grouped into three (3) and treated with cyclophosphamide and Jalin extract. Group one served as control and received distilled water, group two received 100mg/kg of cyclophosphamide per bodyweight of albino rats and group three received cyclophosphamide 24 hours before the administration of 2ml/kg of Jalin extract per bodyweight of albino rats. Blood samples were collected and analyzed for body weight, liver weight, liver to body weight ratio and liver enzymes (AST, ALT and ALP). Results showed a significant (P<0.005) increase in liver enzymes(ALT, AST and ALP). ALT increased from (32.20±1.64) to (96.60±1.02), AST increased from (52.60±1.52) to (110.70±1.17) while ALP (224.23±2.30) to (260.20±2.40) when compared to control. On the other hand, a significant decrease was observed in the level of serum AST, ALP and ALT following treatment with jalin extract and cyclophosphamide when compared to the cyclophosphamide group only. ALT decreased from (96.60±1.20) to (52.60±2.90); AST decreased from (110.70±1.17) to (67.20±1.30) while ALP decreased from 260.20±2.40 to (235.40±3.90) after treatment with jalin extract and cyclophosphamide. Histopathology of the rat liver treated with cyclophosphamide alone showed degeneration of the central vein, necrosis, occluded sinusoidal space and ballooning of hepatocytes. Rats treated with Jalin extract and cyclophosphamide showed normal morphology of the liver, central vein, hepatocytes with intact sinusoidal space similar to the histopathology of control rats. Conclusively, jalin extract is a curative potential herbal remedy against liver dysfunction and should be encouraged.

Keywords: Cyclophosphamide, Jalin extract, Toxicity, Liver.