IN VITRO ANTIDIABETIC ACTIVITY OF ETHANOL EXTRACT OF EEMBELIA RIBES BY GLUCOSE UPTAKE ASSAY BY PERIPHERAL TISSUES
Amir Sohel*, Jha Aditya G. K., Ramesh C., Sowmya B. A. and Pinkey Rawal
ABSTRACT
The present research study was conducted to determine the mechanism of action of Embelia ribes for its antidiabetic properties. Materials & Methods: The authenticated leaves of plant Embelia ribes were dried and powdered. The powdered drug was defatted with petroleum ether and subjected to ethanol extraction. The ethanol extract of Embelia ribes was subjected to preliminary phytochemical investigation. The glucose up takes by rat hemi-diaphragm (skeletal muscle) model was also used evaluate potentials of the ethanol extract of Embelia ribes to enhance utilization of the blood glucose by peripheral tissues. The overnight fasted albino rat was sacrificed and dissected to hemidiaphragm. Weighed quantity of skeletal muscle was incubated with glucose in Tyrode solution for 30 mins at 37oC and the amount of glucose utilized by the tissue was determined. Results: The ethanol extract of Embelia ribes significantly increased the glucose utilization by skeletal muscle which its shows potency to sensitize the insulin. Conclusion: The results of the presents investigation recommends that, one of the mechanism of ethanol extract of Embelia ribes for antidiabetic activity is reduction of insulin resistance.
Keywords: Antidiabetic activity, Embelia ribes, Hemidiaphragm, Glucose utilization.
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