European Journal of Biomedical and Pharmaceutical Sciences

POTENTIAL OF KALAKAI LEAF (STENOCHLAENA PALUSTRIS) EXTRACT AS AEROMONAS HYDROPHILA ANTIBACTERIA AND TOXICITY IN STRIPED CATFISH (PANGASIUS HYPOPTHALMUS)

Ririen Kartika Rini*, Siti Aisiah, Noor Arida Fauzana, Fatmawati, Olga, Aminah, M. Alvin Rifqi

ABSTRACT

Aeromonas hydrophila is a bacteria that infects freshwater fish and causes an outbreak of motile aeromonad septicemia (MAS). One of the natural Borneo herbs that can be used in the control of the MAS, the kelakai leaf (stenochlaena palustris). The research aims to determine the inhibition of extract kelakai leaf on A. hydrophila in vitro and to analyze the toxicity a variety of doses of kelakai leaf extracted on the striped catfish (Pangasius Hypopthalmus). The method used in this study used kelakai leaf extract in vitro discus diffusion method with three solvents namely akuades, methanol and antibiotics as control, followed by toxicity test on striped catfish using random design complete with 6 treatments is K : without the extract kelakai leaf, extracts of kelakai leaf 50 mg/L (A), 100 mg/L (B), 200 mg/L (C), 400 mg/L (D), dan 800 mg/L (E). Antibacterial test extracts from kelakai akuades inhibition zone 11,02 ± 0,27 mm, extracts kelakai ethanol 8,48 ± 0,28 mm, and antibiotics (control) 11,87 ± 0,14 mm. Toxicity test for the survival of striped catfish were obtained K (control) 83,33%, A (83,33%), B (90%), C (96,67%), D (93,33%,) dan E (90 %), the result of Anova test show no difference between treatment of the meaning of all non-toxic doses of extract on striped catfish. It was determined that extracting kelakai leaf would kill a bacterium A. hydrophila and all dose of kelakai leaf akuades extract could be applied in treatment and potentially powerful to develop as a raw ingredient in controlling A. hydrophila infections in freshwater fish.

Keywords: Aeromonas hydrophilla, kelakai, striped catfish, MAS.