European Journal of Biomedical and Pharmaceutical Sciences

CHARACTERIZATION OF PVL (PANTO-VALANTINE LEUCOCIDINE) GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) ISOLATES FROM SOME HOSPITALS IN BAGHDAD CITY

*Norrya. A. Ali, *Ashwak B. AL –Hashimy and *Huda S. Alagely

ABSTRACT

The present study determined the prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) from 210 clinical samples collected from three hospitals Al-kindy, Al-Yarmouk and Ibn Al-balady hospitals. From the beginning of January 2015 till the end of May2015, all samples were bacteriologically examined by Traditional based on cultural characteristics, biochemical reactions using API Staph. and molecular methods using PCR. The result of traditional methods revealed that 150 were S. aureus while 143 isolated depending on molecular methods by using nuc gene .In another hand Methicillin resistance was determined by Kirby-Bauer’s disc diffusion method. The PCR was used for MecA gene detection from MRSA strains, 137were MRSA positive MecA gene and six MRSA strains were MecA negative while, 120 isolates were MARSA positive PVL gene when visualized on 2% agarose gel electrophoresis.

Keywords: aureus, MecA, PVL gene, Antibiotics, Staphylococcal infections, Methicillin resistance.