Ashish D. Lande*, Dhammadip C. Nandgaye, Rakesh Y. Bhute and Dr. Mohammad Tauqeer Shiekh


Hands are the most common mode of transmission of pathogens to patients proper hand hygiene can prevent health care associated infections and the spread of antimicrobial resistances. The aim of present study was preparation and evaluation of herbal neem disindectant.the objective of the study was to prepare and herbal hand sanitizer incorporating the leaves extract of Azadirachta indica (neem)and marketed preparation of castor oil the well known herbal combination with multidimensional activities. The research was carried out to study the antimicrobial properties of aqueous extract of neem leaves using a agar plate diffusion method and effect on microflora. Based upon the result hand sanitizer was prepared using neem oil and castor oil. The hand sanitizer developed screened for its antimicrobial activity .The main goal of herbal hand sanitizer was for hand hygine. It is a vital principle in the prevention, control and reduction of acquired infections. Hand sanitizer avoids adverse effects like itching, irritation, dermatitis, etc. So maintaining hand hygine as the prime criteria instead of same herbal formulations an attempt has been made to formulate as formulation. The formulation had been then tested for its physicochemical properties like foam retention, foam height, ph, spreadiblity and gave satisfactory result. The formulation are found to be very efficacious in all the parameters and all found the enhance antimicrobial property. The antimicrobial activity of the formulated herbal hand sanitizer was tested against S.aureus &E-coli by using cup plate method and the results obtained were compared with commercial antibacterial standards. Overall results revealed that the hand snitizer is efficient in reducing number of organisms as its larger inhibition zone than the marketed synthetic hand sanitizer preparation .it is sure that these ingidents especially mine on combination behave an effective hand sanitizer.

Keywords: Hand hygiene, Antimicrobial activity, Herbal sanitizer, Neem oil, Castor oil.

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