European Journal of Biomedical and Pharmaceutical Sciences

PHARMACOLOGICAL POTENTIAL OF FLAVONOID COMPOUNDS IN ETHANOL EXTRACT ARTOCARPUS HETEROPHYLLUS LAM. LEAVES AS NATURAL ANTIOXIDANTS SOURCES

Adi Parwata I M.O.*, Rica Dwi Adnyani, Sutha Negara I M., Bawa Putra A.A. and Putu Gede Adi Purwahita

ABSTRACT

Artocarpus heterophyllus Lam. is a one of the traditional medicinal plants in Asia. In traditional Chinese medicine, India and Indonesia leaves of Artocarpus heterophyllus Lam are used as antidiabet, antitumor, prevent cancer, fever, prevent osteoporosis, blood circulation, prevent hypertension, strengthen bone and flu medicine. The leaves of Artocarpus heterophyllus Lam. contains high levels of flavonoids (Marianne, 2011). Flavonoids as antioxidants can prevent degenerative diseases such as diabetes, heart disease and cancer. This research aimed to explore the antioxidant of flavonoids in Ethanol Extract Artocarpus heterophyllus Lam. Leaves. Dry powder of Artocarpus heterophyllus Lam. leaves was macerated with ethanol. Liquid extract ethanol was concentrated by rotary evapourator. Consentrated extract was analyzed total flavonoid and antioxidant capacity in vitro than the isolation, identification and antioxidant activity test of its flavonoid. The results obtained are total flavonoid (mg /100 g QE) of ethanol extract = 422 and antioxidant capacity (IC50 = mg/L) = 12,65. These results indicate that ethanol extract has the highest total flavonoids and the strongest antioxidant activity. Based on this result then continued with the isolation, identification and test of antioxidant activity of flavonoid compound on ethanol extract. Identification of isolates by spectroscopy of UV-Vis showed 2 absorption bands. Band I at 378.90 nm and band II at 279,60 nm. This result is thought to be a type flavonoid flavonol. Addition of AlCl3 / HCl reagent showed band I undergoing a batochromic shift. This shows the presence of hydroxy groups in C3’, C4’ and C5 atoms. This isolation and identification results are suspected to be 5,3’,4’-trihydroxy-flavonol. The antioxidant activity test of 5,3’,4’-trihydroxy-flavonol yielded IC50 = 18.67 ppm. These results suggest that 5,3’,4’-trihydroxy-flavonol has very strong antioxidant activity, and potentially developed as a natural antioxidant agent in preventing degenerative diseases.

Keywords: Artocarpus heterophyllus Lam, antioxidant, total Flavonoid and Flavonol.