*Dr. Ghazala Nathu, Dr. Adila Nathu and Saahil Patel


Stem cells are of vital interest to the scientific and medical communities for their promising role in regenerative medicine, avoiding host rejection, and providing curative therapy for a bevy of pathophysiological conditions. However, being able to obtain enough high-quality, versatile stem cells, especially later on in life, is a pressing obstacle that needs to be addressed. It has been observed that certain nutraceuticals have the potential to increase the activation and proliferation of various stem cells. However, it thus far has not been examined whether abdominal liposuction involving physical alterations of adipose fat leads to similar stem cell activation. Specifically, it has not been probed as to whether internal stem cell mobilization occurs as a viable alternative to SVF treatment that exclusively works with the lipoaspirate. Such activity is expected to be the case as liposuction disturbs the interstitial space inhabited by stem cells thereby increasing the presence of stem cells in the peripheral blood following the procedure. To that end, an intensive literature analysis indicates that there are proven and accurate techniques by which to measure the titers of mesenchymal, hematopoietic, and endothelial progenitor stem cells in blood samples obtained from patients. This will rest largely on antibody staining and flow cytometry analysis for quantification purposes. A tentative methodology, supported by precedent, consists of analyzing blood samples periodically over two weeks to gauge maximal stem cell activation, and to support such a hypothesis, various cytokine, growth factor, and endothelial cell counts will also be examined to substantiate the presence of interstitial disruption. The results of this study, whether concordant or discordant with the hypothesis, will provide consequential insights into potential mechanisms to mobilize stem cells for clinical and therapeutic endeavors.

Keywords: nutraceuticals, lipoaspirate, mesenchymal, cytometry.

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