European Journal of Biomedical and Pharmaceutical Sciences

ROLE OF CAMEL’S MILK WITH OR WITHOUT LEISHMANIA ANTIGEN AS IMMUNE IMPROVER AGAINST INFECTION OF LEISHMANIA DONOVANI IN MALE MICE

Basaad A. Al-Aboody, Khalid G. Al-Fartosi*, Amal F. Al-Gorani

ABSTRACT

The present study aimed to evaluate the role of camel's milk with or without leishmania antigen As immune improver against infection of Leishmania donovani in male mice. 120 of mature male mice divided into five groups by 24 mice for each group. The first group was treated by 0.2 ml of normal saline for 15 days and non-injected parasite considered negative control, the second group was treated by 0.2 ml of normal saline for 15 days and injected with 0.2 ml of the parasite dose (1.2 × 106 parasite/ 0.2ml) considered a positive control, the third group treated with 0.2 ml of camel’s milk, the forth group injected by 0.2 ml of leishmania antigen only, the fifth group treated with 0.2 ml of camel’s milk and injected with 0.2 ml of leishmania antigen . The third, fourth and fifth groups were injected with 0.2 ml dose of the parasite after treatment. Body weight was measured before and after the end of experiment. The each group dissected in two periods 45 days and 90days. The weights of liver and spleen was taken to a statement rates of infection in the organs. Blood samples collected to measure the level of malondialdehyde (MDA) and ceruloplasmin (Cp).The results in two turn 45 and 90 days showed a significant increases (P<0.05) in liver weight, lengh of spleen, ratio of spleen enlargement, MDA level and Cp level in second group compared with the first group. The third and fourth groups have shown a significant decrease (p<0.05) in liver weight , length of spleen , ratio of spleen enlargement, MDA level compared with the second group. The fifth group in two turn 45 days and 90 days, showed non a significant differences (p <0.05) in most of the above parameters compared with first group, and indicated a significant decrease (p<0.05)compared with the second, third and fourth groups.

Keywords: Camel’s milk, Leishmania antigen, Leishmania donovani, Male mice