European Journal of Biomedical and Pharmaceutical Sciences

AN APPROACH FOR MEASURING THE RELEASE OF AN ENDOPHYTIC BIOACTIVE OXYLIPIN FROM SOLID LIPID NANOPARTICLES

Mohamed Dawoud*, Randa Abdou

ABSTRACT

Endophytes are considered a promising source of new bioactive secondary metabolites and are found in almost all plants. Lipid nanoparticles are of interest as a carrier for these bioactive metabolites. It is necessary to fully characterize the drug retention and release properties of these nanoparticles loaded with these bioactive metabolites. Ion exchange column technique was used to study drug transfer from such carriers to lipophilic acceptor particles. An endophytic fungus was isolated from the medicinal plant Bidens bipinnata. The transfer of the bioactive metabolite from the negatively donor lipid nanoparticles, which were prepared from trimyristin, to the neutral acceptor unilamellar vesicles was examined after separation between donor and acceptor on an ion exchange column. The ethyl acetate extract of the fungal isolate exhibits antibacterial activity as well as cytotoxic and antiproliferative effects. Activity-guided chromatographic fractionation resulted in the isolation of the active metabolite which was identified as a new bioactive oxylipin. The transfer of this bioactive oxylipin was rapid but it stopped at a concentration less than the expected value of equal distribution. The medicinal plant Bidens bipinnata was chosen for the search of bioactive secondary metabolites due to its anti-inflammatory and antifungal effects. The low equilibrium values might be attributed to the localization of the oxylipin at the interface of the acceptor unilamellar vesicles. Ion exchange column is a suitable technique to study the transfer of the bioactive metabolite oxylipin to acceptor unilamellar vesicles.

Keywords: Ion exchange column, endophytic metabolite, drug transfer, unilamellar vesicles.