Abstract
AN APPROACH FOR MEASURING THE RELEASE OF AN ENDOPHYTIC BIOACTIVE OXYLIPIN FROM SOLID LIPID NANOPARTICLES

Mohamed Dawoud*, Randa Abdou

ABSTRACT

Endophytes are considered a promising source of new bioactive secondary metabolites and are found in almost all plants. Lipid nanoparticles are of interest as a carrier for these bioactive metabolites. It is necessary to fully characterize the drug retention and release properties of these nanoparticles loaded with these bioactive metabolites. Ion exchange column technique was used to study drug transfer from such carriers to lipophilic acceptor particles. An endophytic fungus was isolated from the medicinal plant Bidens bipinnata. The transfer of the bioactive metabolite from the negatively donor lipid nanoparticles, which were prepared from trimyristin, to the neutral acceptor unilamellar vesicles was examined after separation between donor and acceptor on an ion exchange column. The ethyl acetate extract of the fungal isolate exhibits antibacterial activity as well as cytotoxic and antiproliferative effects. Activity-guided chromatographic fractionation resulted in the isolation of the active metabolite which was identified as a new bioactive oxylipin. The transfer of this bioactive oxylipin was rapid but it stopped at a concentration less than the expected value of equal distribution. The medicinal plant Bidens bipinnata was chosen for the search of bioactive secondary metabolites due to its anti-inflammatory and antifungal effects. The low equilibrium values might be attributed to the localization of the oxylipin at the interface of the acceptor unilamellar vesicles. Ion exchange column is a suitable technique to study the transfer of the bioactive metabolite oxylipin to acceptor unilamellar vesicles.

Keywords: Ion exchange column, endophytic metabolite, drug transfer, unilamellar vesicles.


[Full Text Article]   [Download Certificate]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: MAY ISSUE PUBLISHED

    MAY 2024 Issue has been successfully launched on 1 MAY 2024.

  • EJBPS New Impact Factor

    EJBPS Impact Factor has been Increased to 7.482 for Year 2024.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT