PLANTLET REGENERATION VIA CALLUS INDUCTION FROM LEAF EXPLANTS OF SOLANUM SURATTENSE BURM. (F) A MEDICINALLY IMPORTANT PLANT
*Ugandhar T., Odelu G., Ayodhya Ramulu. Ch, Anitha Devi U., Sammaiah D. and Venkateshwarlu M.
ABSTRACT
Solanum surattense Burm (F) a fast growing medicinal plant has been successfully plantlet regeneration via callus
induction from leaf explants under in vitro condition. In this study the Leaf explants of two weeks old seedlings of
S.surattence were induced for callus induction on MS media containing different combinations and concentrations
of growth regulators. Different callusing media containing varying levels of 2.4-D/IAA/NAA (1.0-5.0mg/L) were
tested for callus induction response. Maximum (90%) callusing response was obtained from leaf explants on MS
medium containing (3.0mg/L) NAA. After 8 weeks of induction, the calluses were transferred on different
regenerated media containing varying level of BAP/Kn/TDZ (1.0-5.0mg/L). Maximum shoot bud differentiation
from callus culture was achieved on MS Medium fortified with BAP (3.0mg/L). Elongation and further
development of shoot buds into shoots were achieved on MS medium supplemented with NAA (0.5mg/L) and
BAP (3.0mg/L). This is the first report of in vitro callus induction and plantlet regeneration in S. surattense Burm
(F). The regenerated elongated shoots were transferred to Indole Butyric Acid (IBA) (1.0mg/L–5.0mg/L) for root
induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under
culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants
was 86%. Plants looked healthy with no visually detectable phenotypic variations.
Keywords: Callus induction, Regeneration, BAP: 6-Benzylaminopurine; IAA: Indole-3- Acetic Acid; MS: Murashige and Skoog IBA: Indolebutyric acid, Kn:- Kinetin, NAA: Naphthyl acetic acid.
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