European Journal of Biomedical and Pharmaceutical Sciences

ANTI- INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITY OF JASMINUM AURICULATUM VAHL FLOWER EXTRACT

Leela J.*, Dr. Basavaraj H. and Dr. Mallappa Shalavadi

ABSTRACT

Aim: Aqueous extract of flower Jasminum auriculatum vahl (Oleaceae) was evaluated for anti- inflammatory and anti-arthritic activity using Carrageenan induced paw edema and Complete Freund’s adjuvant induced arthritis model in rats respectively. Materials and Methods: The flowers of the plant Jasminum auriculatum was collected, shade dried and extracted by maceration method using Chloroform water I.P. Aqueous extract was subjected for phytochemical screening, pharmacognostic evaluation and acute oral toxicity as per OECD guidelines (423). Anti-inflammatory activity was tested by measuring change in rat paw edema induced by Carrageenan subcutaneously in rats. Antiarthritic activity was also evaluated by measuring paw volume, arthritic index, and body weight induced by Complete Freund’s adjuvant in rats. The determination of hematological parameters and markers in serum levels of enzymes like SGPT, SGOT, serum RF and histopathological studies also conducted. Results: phytochemical screening revealed the presence of alkaloids, flavonoids, terpenoids, phenols and tannins as active constituents. In acute oral toxicity study, the AEJA has produced no lethality with a maximum dose of 2000 mg/kg body weight hence 1/5th, 1/10th and 1/20th mg/kg body weight were taken as lower, moderate and higher dose respectively. AEJA at concentration of 100, 200 and 400 mg/kg was able to reduce the inflammation induced by carrageenan in rats. However, 200mg/kg, 400 mg/kg has shown significant inhibition of arthritis in rats when challenge with CFA (0.1 ml) in rats. The AEJA of dose of 200 mg/kg, 400 mg/kg has maintained normal architecture of Bone, normal (areolar) Synovium and normal histology as compared to arthritis control. Conclusion: Our study concludes that the AEJA possess anti-oxidant, anti-inflammatory and anti- arthritic activity in a dose dependent manner. The activity of extract may be due to the presence of phytochemicals i.e., flavonoids, terpenoids and phenols as active constituents.

Keywords: Inflammation, RA-Rheumatoid arthritis, Complete Freund’s adjuvant, Carrageenan, AEJA-Aqueous extract of Jasminum auriculatum.