TUMOR INHIBITORY L-ASPARAGINASE PRODUCTION BY ASPERGILLUS CERVINUS
Praveen Kumar Dasari*, Bonagiri Vamsi Krishna, Gottapu Govardhini, Mohammad Raisa Fathima, Mukara Bhaskar Rao, Sadhu Sowmya and Tanuku Satyanarayana
ABSTRACT
L – asparaginase is an important therapeutic enzyme used for the treatment of acute lymphoblastic leukemia, lymphosarcoma, lymphoproliferative disorders and acute lymphocytic leukemia. The present research work discribes the production of L-asparaginase using Artocarpus heterophyllus as substrate by Aspergillus cervinus in a solid state fermentation. Normally L-Asparaginase enzyme existed in many animal tissues, plant tissues and bacterial cell, but not in human cells. In the present research, L-asparaginase enzyme production was carried out by observing different parameters like incubation time, incubation temperature, pH, inoculum level and moisture content were noted. Different carbon supplements were checked for their influence on enzyme production; they are glucose, sucrose, maltose, fructose, and lactose. The incubation time of 72hrs, the temperature of 360C, pH 5.0, inoculum level of 70% v/w and moisture content of 70% v/w were observed for L-asparginase enzyme production. Among the carbon source, sucrose gave better production when compared with other carbon supplements. Nitrogen source like L-asparagine with different concentrations were checked and 0.4% w/w gave best enzyme production. Final conclusion is that Artocarpus heterophyllus could be a promising substrate for industrial application since it produces a significant L–asparaginase (89.36 IU/ml) activity in solid state fermentation.
Keywords: Artocarpus heterophyllus, Aspergillus cervinus, Solid-state fermentation.
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