Praveen Kumar Dasari*, Bonagiri Vamsi Krishna, Gottapu Govardhini, Mohammad Raisa Fathima, Mukara Bhaskar Rao, Sadhu Sowmya and Tanuku Satyanarayana


L – asparaginase is an important therapeutic enzyme used for the treatment of acute lymphoblastic leukemia, lymphosarcoma, lymphoproliferative disorders and acute lymphocytic leukemia. The present research work discribes the production of L-asparaginase using Artocarpus heterophyllus as substrate by Aspergillus cervinus in a solid state fermentation. Normally L-Asparaginase enzyme existed in many animal tissues, plant tissues and bacterial cell, but not in human cells. In the present research, L-asparaginase enzyme production was carried out by observing different parameters like incubation time, incubation temperature, pH, inoculum level and moisture content were noted. Different carbon supplements were checked for their influence on enzyme production; they are glucose, sucrose, maltose, fructose, and lactose. The incubation time of 72hrs, the temperature of 360C, pH 5.0, inoculum level of 70% v/w and moisture content of 70% v/w were observed for L-asparginase enzyme production. Among the carbon source, sucrose gave better production when compared with other carbon supplements. Nitrogen source like L-asparagine with different concentrations were checked and 0.4% w/w gave best enzyme production. Final conclusion is that Artocarpus heterophyllus could be a promising substrate for industrial application since it produces a significant L–asparaginase (89.36 IU/ml) activity in solid state fermentation.

Keywords: Artocarpus heterophyllus, Aspergillus cervinus, Solid-state fermentation.

[Full Text Article]   [Download Certificate]


Forgot Password  |  Register


Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation


    MARCH 2024 Issue has been successfully launched on 1 MARCH 2024.

  • EJBPS New Impact Factor

    EJBPS Impact Factor has been Increased to 7.482 for Year 2024.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.


  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.


UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS


Copyright From

Covering Letter

                        Author Instruction