European Journal of Biomedical and Pharmaceutical Sciences

COMPARATIVE HPTLC FINGERPRINTING STUDY OF SHODHANA OF VATSANABHA (ACONITUM CHASMANTHUM STAPF EX HOLMES) FROM TWO DIFFERENT MEDIA

*Dr. Meenu Bharti Sharma, Prof. Dr. Upendra U. Zala and Dr. Ankit M. Paneliya

ABSTRACT

Introduction: Ashuddha Vatsanabha (Aconitum Chasmanthum Stapf Ex Holmes) is very toxic in nature. For therapeutic use, Vatsanabha can be use after Shodhana only. In this study, two different kind of media (Gomutra & Godugdha) were taken to purify Ashuddha Vatsanabha. Now, to see any changes after Shodhana as well as comparison of two media were done. For that, we have compare the HPTLC fingerprinting study to know the presence of compoments and comparison of more components. HPTLC is a sophisticated and automated form of TLC. With HPTLC, the analysis can be viewed using different wavelengths of light thereby providing a more complete picture of the plant than is typically observed with more specific types of analyses. Material and Methods- Principle of HPTLC, steps involved in procedure using specify techniques for chromatography. Results and Observation- After Derivatization, plate was examined visually for appearance of different bands at different Rf (Rf254 nm, Rf366 nm Rf 540 nm). Discussion- At different wavelength, tracks were having no. of spots. In all tracks, they showed the presence of components but some were having value showed the presence of same component. Some, were having different spots resulting in presence of another component also. Conclusion- Gomutra Shodhita Vatsanabha – GMSV Powder (Track 2) showed more components in respective of all wavelengths as comparison to Godugdha Shodhita Vatsanabha –GDSV Powder (Track 3). So, we can conclude that Vatsanabha Shodhana if done by Gomutra media can have more active constituents and other properties results in efficacy also than Godugdha media.

Keywords: In this study, two different kind of media (Gomutra & Godugdha) were taken to purify Ashuddha Vatsanabha.