ANTIUROLITHIATIC ACTIVITY OF NEEM LEAVES IN EXISTING RENAL CALCULI BY INVITRO METHODS.
Malla Sudhakar*, Ramya Kumari B.S, Poornashree M., Ankitha Gour, Sahani Sultana and Saroj Mahala
ABSTRACT
Kidney stones or calculi are most prevalent diseases around the world. Calcium oxalate was found to be the most
important component of the calculi. Most of the ayurvedic practitioners use ethanobotanical herbs to cure these
calculi. But as such no such scientific evidence is available till date on these plants. The present study was
focussed mainly to study the inhibitory effects of the A.indica (Neem plant) on the stone formation. Neem leaves
ash was used to demonstrate the invitro activity on the stone formation. Leaves ash was pulverized and was
extracted with solvents like Methanol, Chloroform and Water. The invitro activity was performed to estimate the
inhibitory activity of these extracts on the formation of calculi. NEERI and Cystone were used as positive control.
Antiurolithiatic activity was studied by titrimetry and turbidity methods. Methanol extract of sample was proved to
competent enough with the standard drugs, cystone and NEERI in dissolving the existing calcium oxalate stones.
Methanol, Chloroform and water extracts showed 53%, 38% and 32% of dissolution of stones respectively. And
Cystone and NEERI showed 65% and 62% respectively. Maximum inhibition in calculi formation (turbidity) was
seen by the methanol extract after 210 seconds of chemical reaction as compared to the control, Cystone and
NEERI. Chloroform and water extracts both showed similar activity, but still chloroform extract being the second.
At present Cystone and NEERI were prescribed by medical practioners as effective medicine for preventing and
treating the calculi formation. The present study confirms of the activity of our sample in comparison to the standard drugs. Further we plan to study the properties in detail and try to optimize the parameters to make it more
efficient in treating the calculi.
Keywords: Calculi, Calcium oxalate, Turbidity method, Neem leaves, Cystone.
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