Abstract
PRODUCTION OF L-METHIONINE AS A FUNCTION OF UTILIZATION OF CARBON AND NITROGEN BY A MULTIPLE ANALOG-RESISTANT MUTANT Corynebacterium glutamicum X300

Subhadeep Ganguly and *Kunja Bihari Satapathy

ABSTRACT

An experimental study was conducted to examine the pattern of changes in the cell mass, glucose utilization and changes in the nitrogen contents in the broth during L-methionine fermentation by a multiple analog-resistant mutant Corynebacterium glutamicum X300. Utilization of glucose increased gradually along with the gradual increase of dry cell mass. L-methionine content increased upto 72h, then decreased gradually due to excessive cellular population which might adversely affect L-methionine production. Amino nitrogen level increased upto 72h as it is an indicator of L-methionine production. Cellular nitrogen and ammonical nitrogen increased gradually through out the study with sharp decline in residual glucose level.

Keywords: Experimental, Corynebacterium glutamicum X300, L-methionine, glucose.


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