European Journal of Biomedical and Pharmaceutical Sciences

DEVELOPMENT OF NEW ANALYTICAL METHOD FOR ESTIMATION OF CALCIUM CHANNEL BLOCKER AZELNIDIPINE

Prakash S. Sarsambi*, Ramesh D. Avute and Preeti J. Jadhav

ABSTRACT

Azelnidipine is a third generation and long acting dihydropyridine calcium channel antagonist used for effective antihypertensive agent. Three simple, sensitive, accurate and economical spectophotometric methods have been developed for the estimation of Azelnidipine in bulk drug and pharmaceutical formulation. Method A is based on UV-spectophotometric measurements in which Azelnidipine was dissolved in 0.1N HCl and exhibited absorption maxima at 220nm and obeyed Beer‟s law in the concentration range of 5-30μg/ml. In Method B and C the amine group of reduced Azelnidipine forms colouredchromogens. In method B Azelnidipine forms colouredschiff‟s base with PDAC(Para- Dimethyl amino cinnamaldehyde) reagent to yield orange red colouredchromogen exhibitingabsorption maxima at 530nm and obeyed Beer‟s law in the concentration range of 20-100μg/ml. In Method C the reduced Azelnidipine is diazotized with sodium nitrite and HCl to form a diazonium salt and coupled with BMR(Bratton Marshal reagent) i.e.N-1-[Napthylethylenediaminedihydrochloride] reagent and exhibited absorption maxima at 557nm and obeyed Beer‟s law in the concentration range of 20-100μg/ml. Results of the analysis were validated for accuracy, precision, limit of detection (LOD), Limit of quantification(LOQ) were found to be satisfactory. The proposed methods are simple, sensitive, rapid economical and suitable for the routine quality control application in pharmaceutical formulations.

Keywords: Azelnidipine, 0.1N HCl, PDAC, BMR.