European Journal of Biomedical and Pharmaceutical Sciences

EXTRACTION PHYTOCHEMICAL SCREENING AND IDENTIFICATION OF NATURAL PIGMENTS FROM MEDICINAL PLANTS BY THIN LAYER CHROMATOGRAPHY

Dorasala Lakshmi*, Syed Sumaya, Derangula Hari Kumar, Sadda Eswar Reddy and Chadalavada Swathi

ABSTRACT

Preliminary qualitative phytochemical analysis made for the leaves of Coriandrum Sativum and Rhizome of Curcuma Longa revealed that presence of glycosides, saponins, fats and oils, sterols and flavonoids. So, these species are expected to have many medical uses. TLC offers several benefits over the other chromatographic separation. The biggest advantage offered to the chromatography is time saving when compared to other chromatographic techniques. The non-volatile compounds can be separated by this method, where in gas chromatography and other chromatographic techniques only volatile compounds can be separated. All components of UV light are achieved to visualize microlite „quantity of sample can also be separated in TLC. TLC proved to be successful method of identifying unknown compounds and determining the polarity of the substance. The rf value helps to identify the phytochemical constituents present in the chloroform extraction of Coriandrum Sativum leaves and Curcuma Longa rhizome. The present study was designed to extract, identify the phytochemically active constituents in rhizome of Curcuma Longa and leaves of Coriandrum Sativum.

Keywords: Extraction, Maceration, Thin layer chromatography, Curcuma, Coriandrum Sativum.