EXTRACTION PHYTOCHEMICAL SCREENING AND IDENTIFICATION OF NATURAL PIGMENTS FROM MEDICINAL PLANTS BY THIN LAYER CHROMATOGRAPHY
Dorasala Lakshmi*, Syed Sumaya, Derangula Hari Kumar, Sadda Eswar Reddy and Chadalavada Swathi
ABSTRACT
Preliminary qualitative phytochemical analysis made for the leaves of Coriandrum Sativum and Rhizome of
Curcuma Longa revealed that presence of glycosides, saponins, fats and oils, sterols and flavonoids. So, these
species are expected to have many medical uses. TLC offers several benefits over the other chromatographic
separation. The biggest advantage offered to the chromatography is time saving when compared to other
chromatographic techniques. The non-volatile compounds can be separated by this method, where in gas
chromatography and other chromatographic techniques only volatile compounds can be separated. All components
of UV light are achieved to visualize microlite „quantity of sample can also be separated in TLC. TLC proved to be
successful method of identifying unknown compounds and determining the polarity of the substance. The rf value
helps to identify the phytochemical constituents present in the chloroform extraction of Coriandrum Sativum leaves
and Curcuma Longa rhizome. The present study was designed to extract, identify the phytochemically active
constituents in rhizome of Curcuma Longa and leaves of Coriandrum Sativum.
Keywords: Extraction, Maceration, Thin layer chromatography, Curcuma, Coriandrum Sativum.
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