TOXIC IMPACTS OF THIODAN 35 EC® ON PROTEIN, GLYCOGEN AND OXYGEN CONSUMPTION IN TILAPIA MOSSAMBICA
Rita Ganava*, Reena Ganava, R. R. Kanhere and Hemant Pandit
ABSTRACT
Endosulfan is one of the most disputed toxic pesticides and is responsible for many severe pesticide poisoning
incidents around the world. The present study is aimed to find out the effects of this pesticide on whole body
oxygen consumption and protein and glycogen content of the liver a fresh water fish, Tilapia mossambica. The
toxicity tests were conducted by static renewal bioassay method on the juveniles of fish Tilapia mossambica
(Peters). The probit analysis showed that the LC50 (lethal concentration) value of endosulfan (Thiodan 35 EC® )
for 24, 48, 72 and 96 h were 0.9, 0.5, 0.1 and 0.05ppm for 96 hours. The teleost fish Tilapia mossambicus of
15±1g was exposed to three different sub lethal concentrations of Thiodan 35 EC® for 24,48,72,96 hours. Hepatic
metabolic parameters like protein and glycogen were also determined. Results clearly shown that rate of oxygen
consumption were declined along with the increase of dose and duration. Decrease in both in respiratory rate and
in body weight (P<0.05) was also observed in all treated groups. One third (30 μg/L) one tenth (5 μg/L) and one
fifth (10 μg /L) of the LC50 values were selected for sub lethal studies. An initial increase in the oxygen
consumption was observed in low sub lethal concentrations (1/5th and 1/10th exposure) but thereafter a sharp
decrease in oxygen consumption was observed in all sub lethal concentrations. Results also clearly revealed that
protein, glucose and glycogen content of liver were significantly reduced in all experimental groups treated with
sub lethal concentrations of endosulfan. The reduction in glycogen content of liver in all the experimental fishes
might be due to the utilization of carbohydrates for energy production as a result of toxicant induced hypoxia was
observed which has also been reflected in reduction in oxygen consumption. Fish under sub lethal concentration
were found to be under stress and duration of the exposure is found to be an important factor for inducing the toxic
effects. Hence, respiratory dysfunction and hepatic metabolic markers like glucose and glycogen can be used as an
index of Parathion toxicity.
Keywords: Pesticide, Endosulfan, glycogen, Tilapia mossambica, oxygen consumption.
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