European Journal of Biomedical and Pharmaceutical Sciences

UFLC SIMULTANEOUS DETERMINATION OF PARACETAMOL PANTAPRAZOLE AND ACECLOFENAC IN BULK AND BY COMBINING THE DOSAGE FORMS

Dr. Sreejanardhanan V. and *Shine Sudev

ABSTRACT

An optimized UFLC method was successfully developed and validated for the detection and quantification of paracetamol, pantoprazole, and aceclofenac in both bulk and tablet formulations. the method is simple, accurate, rapid, and precise. reverse phase ODS column was utilized for separation, and a mixture of phosphate buffer (ph 4.5) and acetonitrile in the ratio of 40:60 was used as the eluent. the flow rate was maintained at 0.7 ml/min, and detection was performed using a uv spd20 detector at 284 nm, while the ambient temperature was kept constant. the proposed method was optimized and validated in accordance with the ich guidelines, making it suitable for routine quality control analysis. overall, this method provides a reliable and efficient means of analyzing these compounds in a variety of settings.

Keywords: UFLC, Paracetamol, Pantaprazole, Aceclofenac, Method development.