EXTRACTION AND STUDY ON CARISSA SPINARUM FRUIT EXTRACT AS NATURAL INDICATOR ALTERNATIVE TO SYNTHETIC INDICATOR
Sivalalitha. Pyla, *B. Nagamani, Suvarna Kasi and P. Umadevi
ABSTRACT
Aim: The aim of the present study is focused on to extract and study carissa spinarum fruit extract as natural
indicator alternative to synthetic indicator Indicators are the substances that changes color when they are added to
acidic or alkaline solutions. These help to determine the sharp endpoint of any compound by changing its color
based on their pH. Synthetic indicators are the contemporary chemistry regarding environmental pollution. The
present work highlights the use of the extract of the flowers, leaves and fruits, of Carissa Spinarum used as a
natural indicator in acid-base titrations. It shows sharp color at endpoint. Indicators are dyes or pigments that can
be isolated from various sources like plants, fungi and algae. Synthetic indicators give accuracy results but are
non-ecofriendly, unsafe, and detrimental. Some phytochemical analysis shows anthocyanin and flavonoids
properties in Carrisa spinarum(ripe fruit) also called Conker berry. We developed this fruit extract as natural
indicator by using 90% ethanol. By using acid-base titration with HCl, NaOH, NH4OH, CH3COOH and also
performed assays on Aspirin, Ammonium chloride, Sodium bicarbonate. In addition, this natural indicator was
also used as universal indicator by comparing with synthetic indicator which had given equivalent sharp color
change and accurate results. Therefore, the natural indicators are found to be eco-friendly, easy available, less cost,
non-toxic and very useful.
Keywords: Carissa spinarum, Indicator, Titration, assays.
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