PROBIOTIC EFFECT OF BACILLUS COAGULANS, MBTU-P1F2 FROM INFANT FAECES WITH A KNOWN PROBIOTIC
Honey Chandran C., Summaya Jauhara and Dr. Keerthi T. R.a*
ABSTRACT
Comparative evaluation of Probiotic potency of Bacillus coagulans, MBTU-P1F2 from infant faeces with a known probiotic Bacillus clausii from Enterogermina provides a new area for searching potent spore former from human intestinal flora. Aim of the study was to isolate and characterize spore forming bacteria from infant faces and comparatively evaluate its probiotic potentials with a known probiotic (Bacillus clausii). The isolate were shown all the essential properties of probiotics such as tolerant to acidic pH, bile salts and viable in simulated gastric juice which would enable them to withstand the conditions prevailing in the gastro intestinal tract and molecularly identified as Bacillus coagulans. Comparative study with Bacillus clausii revealed that Bacillus coagulans, MBTU P1F2 showed more inhibitory against selected pathogens. Cell surface properties of the isolate revealed the presence of hydrophobic components on the cell surface and as in comparision, Bacillus clausii showed more hydrophobicity than MBTU-P1F2. The hydrophobic nature can be related to their ability to adhere to gastrointestinal mucosal epithelium. The studies further proved that they were autoaggregative and coaggregates the main causative agent of enteric fever, Salmonella typhi. MBTU-P1F2 showed capability to deconjugate bile salts (Bile Salt Hydrolase activity, have role in cholesterol removel) and was not detected in Bacillus clausii. The findings proved the probiotic potency of known probiotic Bacillus clausii as well as clear implications for future research in the field, to develop our isolate as probiotic for human or animal nutrition.
Keywords: Probiotics, Human infant faeces, Bacillus coagulans MBTU-PIFI, Enterogermina, Bacillus clausii, Nutrition.
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