ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES IN TERPENOIDS FROM CAMEROONIAN OCIMUM CANUM SIMS. AND XYLOPIA AETHIOPICA (DUN.) A. RICH#
NA Vyry Wouatsa, Amreen A Siddique, Laxminarain Misra* and R. Venkatesh Kumar
ABSTRACT
Bioassay-guided fractionations were carried out on the extracts of aerial parts of Ocimum canum and fruits of Xylopia aethiopica. From the methanol extract of Ocimum canum, a new nerolidol derivative, 6,10-dimethyl-2-methylene-5,7,10-trihydroxydodeca-2,11-diene, was isolated along with eight known compounds, viz. salvigenin, nevadensin, valaran-4,11-diol, lupeol, 24-ethylcholest-4-ene-3-one, 4’-methoxyapigenin, β-sitosterol and stigmasterol. The extract of Xylopia aethiopica afforded mainly known diterpenes kauranol and xylopic acid. The structures of the new and known compounds were elucidated with the help of various spectroscopic techniques and chemical modifications. Some fractions of both the plants possessed a moderate antibacterial activity against all the bacteria tested viz. Gram positive (M. luteus, S. aureus) and Gram negative (K. pneumoniae, P. aeruginosa and S. typhimurium). When tested for their cytotoxic potential, only one fraction displayed a significant cytotoxicity against breast cancer MCF-7 and liver cancer WRL-68 cell lines with IC50 of 9.8 and 8.6 μg/mL, respectively. Therefore, the results of the antimicrobial and cytotoxicity activities performed on the extracts, fractions and isolated compounds from both plants showed a possible synergism among the constituents.
Keywords: Ocimum canum, Xylopia aethiopica, bioassay-guided fractionation, 6,10-dimethyl-2-methylene-5,7,10-trihydroxydodeca-2,11-diene, cytotoxicity, antimicrobial activity.
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